Moina
Moina macropoda (by Peter Brown BKA 484)

Introduction
In the UK Moina is often referred to as Japanese Daphnia and in the USA Russian Red Daphnia. It is closely related to Daphnia species but is much smaller. When they are newly hatched, they are about 0.4 mm with males eventually reaching around 1.13 mm and females 1.65 mm. Moina have one of the highest protein contents of all cultured live foods. They are rich in vitamins A and D and depending on their food source small amounts of vitamins B and C. They can survive temperatures between 4-35 C but I find that a temperature of 24-26 C is ideal. Moina reproduce by live birth or eggs which drop into the bottom sediment. If the female has not been fertilized, she will drop eggs in a special pouch which hatch immediately and develop as females (parthenogenic reproduction). Every 3-5 days a female will produce 20 young, in a further 3-5 days the mother and young will produce offspring! This cycle continues until adverse conditions occur (overfeeding, underfeeding, overcrowding, poor lighting etc). Now, some of the hatching eggs will develop into males. These will mate with the females and cause them to lay resting eggs. These are protected in thick resistant brood pouches which can survive drought and be spread over wide areas. When conditions are suitable, these will hatch as females and the cycle will repeat.
I first became interested in Moina in 1981. Hans van Es contributed an interesting article on culturing it in December’s Killi-News and offered to obtain kits for interested members. Several friends began culturing Moina with reasonable success and I obtained a culture. It soon became apparent that a healthy productive culture one day could crash and die out days later. Eventually Moina seemed to disappear in the BKA and I had to return to weekly daphnia gathering trips …
Searching the internet a couple of years ago I discovered Green Water Farm products in Thailand (https://greenwaterfarmthailand.com). Among other things, they were selling Moina eggs with well detailed raising instructions. I ordered some and the eggs arrived quickly, with well tracked postage in two small, plastic capsules. I hatched ½ of one of the capsules in a 2cm depth of mature aquarium water in a small, well lit plastic tank. The young hatched in a couple of days and were smaller than newly hatched brine shrimps. I fed these on the weak solution of bakers yeast suggested in the instructions and they thrived. In a short space of time I was able to set up several culture jars adding a couple ofsmall Ramshorn snails to each to clean up settled out yeast. I fed enough yeast solution, twice a day to just ‘tint’ the water. Once a week each jar was poured through a brine shrimp net and replaced together with mature aquarium water. This always resulted in the release of lots of you
ng. Occasionally a jar would fail, but having several ‘back ups’ overcame this problem. In addition to clearing up uneaten food the snail droppings contributed infusoria and became covered in algae which helped to keep the water healthy.


A couple of years on I feel reasonably confident in my methods. I like to keep a couple of large jars going, but tend to use 3 litre Hagen plastic tanks for culturing. These are half filled with mature aquarium water. I keep them at the top of the fish room close to the lights (10-12 hours) where they are kept at 24-26C. The feeding solution is made up by dissolving 1 level teaspoon of dried baker’s yeast in 600 ml of mature water. I feed 15ml of this to each plastic tank twice a day as the water clears. It is important to feed from the cultures regularly as this encourages the cycle prevents overcrowding which can lead to the sudden collapse of a culture. The Face Book Group – ‘Live Food Cultures For Aquariums’ has a wealth of interesting information relating to Moina and ideas for larger scale production. I would urge anyone looking for a clean, convenient healthy live-food to experiment with Moina, my Nothos., from fry to adults all love it, the range of sizes always available really helps to move them on.
Moina macrocopa – The Japanese Daphnia Hans van Es – original article Killi-News 196 Dec.1981
When I visited some of my BKA friends in 1978, I heard about a food problem that some members were having which perhaps explained why so many different live foods are bred in the U.K. More than for instance in Holland where live food is plentiful and can easily be caught or even just bought in the shops.
While in England I heard about the Discovery of a new Daphnia in Japan. It was said to be easy to breed and a very good food for the fish, but no more was known about it. It was in 1980 that I again heard about this food and this time got an address from where it could be obtained. I ordered a set and after a very difficult and disappointing start, my results were so good that I think the information should become more common knowledge.
Moina macrocopa, the official name of this fresh water organism, is a creature that multiplies ~sexually by parthenogenesis. An adult produces between 6 and 15 young every 2-4 days, dependent upon the temperature. Below 42°F is fatal for this Daphnia; good growth and multiplication being observed at temperatures from 65°F. The best temperature range is 65-76°F, but higher temperatures of up to 96°F is allowable. At the higher temperature it leads to mass production followed by the rapid distruction of the complete culture and for this reason it cannot be recommended.
Setting up the culture is in fact the most difficult thing to do. In a small amount of water, a little algal nutrient salt is added. To this some moist soil or preferably algae from a tank is introduced. This is then well lit and after about a week the water is poured off.
Fresh water without the nutrient salt is then added to the algae again and now the eggs from the Moina can be added. At a good temperature and well lit, they will hatch within a week and can be seen moving against the light. N
ow the time has come to start feeding the culture, which is achieved with yeast. As it is easy to use, I prefer dried yeast. Just a little of this is added at one time – preferably just sufficient for the Daphnia to consume within 24 hours, but at most 72 hours. Slowly the water level can be raised until it is between 2 and 4 inches.
All this time the tank must be kept under a good light to keep the algae growing, as it is this which supplies the oxygen and also removes the pollutants in the water.
This food is easy to keep, clean, productive, and very easy to feed to the fish. What concerned me when I first acquired this food, was how the fish would react to it. To find out about this I took a batch of three day old fry of Cynolebias (Opthalmolebias) constanciae and divided it exactly in two. One half I raised in the normal way in which I raise fry, but the other half got the Moina immediately they were large enough to consume it. From this time on the growth rate doubled and by the time they were fully grown the fish in the other tank which had not had this food were only half their size. In addition, these larger fish were of a much better quality and proved to be noticeably more productive. This same experiment was tried on several more batches, not just of Cynolebias (Opthalmolebias) constanciae but other species as well, and produced the same results.
The great advantage in feeding this food is that it can be introduced to tanks in fairly large quantities as it causes almost no pollution. All it takes to keep a supply available is to add a little food, which is no more time consuming than. feeding a tank of fish. Cleaning the culture with a good algae vegetation is only very occasionally needed.
The production of a tank with 10 litres of water at a temperature of 69°F is approximately 2 gramms per day – quite a lot that will feed quite a few fish!
Obtaining this Daphnia from the source is unfortunately a very difficult matter as he is having difficulty in getting many parcels through the customs. In addition handling a large number of orders also seems to cause quite a few problems. I was asked therefore to collate orders from Holland and place one large order. I propose also to include in this order members of the BKA. Anyone interested can order a set (no longer than one month after this article appears) by sending £5.00 to me – cash only please! As soon as enough orders have been received I will get the sets and send them as soon as they arrive. If I do not get sufficient orders then I will have to refund the cash. Groups can gather orders together and order in bulk and in this way it might be possible to reduce the price and give a refund.
A breeding set consists of:-
Moina eggs in a vial which can be stored at a temperature of 42°F approximately for many years
Algal nutrient salt
Breeding instructions (in English).
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